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Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Fairchild’s Mule was obtained by crossing Rose-

A) Horse and Ass
B) Potato and tomato
C) Sweet William and Carnation
D) Wheat and Rye
Fairchild's Mule is a well-known example of an intergeneric hybrid in horticulture. It was created by crossing two different genera within the family Caryophyllaceae: the genus Dianthus (specifically the species Dianthus caryophyllus, known as the Carnation) and the genus Dianthus (specifically the species Dianthus barbatus, known as Sweet William). Because these parents belong to different species/genera with different chromosome numbers, the resulting hybrid is sterile, much like a mule (the hybrid of a horse and a donkey), which is why it is named 'Fairchild's Mule'. Analysis of other options: - Option (a) Horse and Ass: This is the biological definition of a literal mule, not a plant hybrid. - Option (b) Potato and tomato: While both are in the Solanaceae family, their hybrid is known as a 'Pomato', not Fairchild's Mule. - Option (d) Wheat and Rye: The hybrid between wheat and rye is known as 'Triticale'.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Match List I with List II: List I: A. Mouldboard plough, B. Chisel plough, C. Rotavator, D. Offset disc harrow List II: I. Operates at depth 45-75 cm, II. Uses rotating blades, III. sets of rotating discs mounted on a common shaft, IV. Inversion of furrow slice

A) AIV, BII, CIII, DII
B) AI, BIV, CI, DIMI
C) AI, BLCIV, DI
D) AIV, BI, CII, DIII
To solve this matching question, we analyze the function and characteristics of each piece of farm machinery listed in List I: 1. **Mouldboard Plough (A):** The primary function of a mouldboard plough is to cut, lift, and completely turn over (invert) the soil slice to bury weeds and crop residue. Therefore, A matches with **IV (Inversion of furrow slice)**. 2. **Chisel Plough (B):** Unlike the mouldboard plough, the chisel plough is used for deep tillage (primary tillage) without inverting the soil. It is designed to break hardpan and operate at significant depths, typically ranging from 30 to 75 cm depending on the model. Therefore, B matches with **I (Operates at depth 45-75 cm)**. 3. **Rotavator (C):** A rotavator (rotary tiller) is a powered implement that uses a series of rotating blades (tines) to break up and mix the soil. Therefore, C matches with **II (Uses rotating blades)**. 4. **Offset Disc Harrow (D):** A disc harrow consists of sets of concave steel discs mounted on a common shaft (gangs) that rotate as the implement is pulled, used for secondary tillage and breaking clods. Therefore, D matches with **III (sets of rotating discs mounted on a common shaft)**. Matching the pairs: A-IV, B-I, C-II, D-III. This corresponds exactly to option (d).
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Multiple factor hypothesis or polygenic inheritance was proposed by-

A) GJ. Mendel
B) Luis de Vilmorin
C) Nilsson-Ehle
D) Hugo de Vries
The Multiple Factor Hypothesis, which describes the inheritance of quantitative traits (polygenic inheritance), was proposed by the French plant breeder Luis de Vilmorin in the 19th century. He observed that traits like seed weight or plant height in peas showed continuous variation and suggested that these traits were controlled by multiple genes, each having a small, additive effect. Analysis of other options: - a. GJ. Mendel: Proposed the laws of segregation and independent assortment, focusing on qualitative traits (monogenic inheritance). - c. Nilsson-Ehle: He provided the genetic basis and experimental proof for the Multiple Factor Hypothesis through his work on kernel color in wheat (1909), but he did not propose the initial hypothesis. - d. Hugo de Vries: Known for the Mutation Theory of evolution, not the Multiple Factor Hypothesis.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

The common toll free number for Kisan Call Centre is-

A) 1800-180-1661
B) 1800-180-1551
C) 1800-180-1771
D) 1800-180-1881
The Kisan Call Centre (KCC) is a critical agricultural extension initiative by the Government of India. It serves as a toll-free helpline for farmers to receive expert advice on various agricultural issues, including crop management, pest control, and livestock care. The official and common toll-free number designated for the Kisan Call Centre across India is 1800-180-1551. Options a, c, and d are incorrect as they do not correspond to the official KCC helpline number.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

The Principle of Equi-Marginal Returns-

A) explains regional specialisation in the production of commodities
B) guides in the allocation of resources under conditions of scarcity
C) explains how losses can be minimized during the periods of price adversity
D) guides in the determination of optimum combination of enterprises
The Principle of Equi-Marginal Returns (also known as the Law of Equi-Marginal Utility or the Principle of Maximum Returns) is a fundamental concept in economics used for resource allocation. It states that a rational decision-maker will allocate resources among various alternative uses such that the marginal return (or utility) derived from the last unit of resource spent on each alternative is equal. 1. Why Option (b) is correct: In agricultural economics, resources (land, labor, capital) are scarce. To maximize total profit or utility, a farmer must allocate these scarce resources across different crops or enterprises so that the marginal return from the last unit of resource is the same for all enterprises. If one enterprise yielded a higher marginal return than another, the farmer would shift resources to that enterprise until the returns equalized. 2. Why Option (a) is incorrect: Regional specialization is primarily explained by the Theory of Comparative Advantage (Ricardian theory), which focuses on the relative efficiency of different regions in producing specific commodities. 3. Why Option (c) is incorrect: Minimizing losses during price adversity is typically handled through risk management strategies, hedging, crop insurance, or diversification, not by the equi-marginal principle. 4. Why Option (d) is incorrect: While the principle helps in the process, the 'determination of optimum combination of enterprises' is the broader goal of Farm Management planning. The Principle of Equi-Marginal Returns is the specific *mechanism* or *guide* used to achieve that allocation under scarcity.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

A pureline variety is obtained from-

A) Cross pollinated crops
B) Vegetatively propagated crops
C) Self-pollinated crops
D) Both self and cross pollinated crops
A pureline is the progeny of a single, homozygous plant. To obtain a pureline, a plant must be genetically uniform (homozygous) across all loci. In self-pollinated crops, repeated selfing (self-pollination) leads to an increase in homozygosity; after several generations, the plant becomes a 'pure line' where all offspring are genetically identical to the parent. Why other options are incorrect: - a. Cross-pollinated crops: These crops typically maintain high heterozygosity due to outcrossing. While they can be made homozygous through repeated selfing, the resulting 'inbred lines' often suffer from inbreeding depression, making them unsuitable as standard 'pureline varieties' in the same sense as self-pollinated crops. - b. Vegetatively propagated crops: These are clones. While they are genetically identical, they are often highly heterozygous. A clone is not a 'pureline' because a pureline specifically refers to a homozygous lineage derived from sexual reproduction (selfing). - d. Both self and cross pollinated crops: Since the fundamental definition of a pureline relies on the achievement of homozygosity through selfing without the detrimental effects of inbreeding depression (which occurs in cross-pollinated species), it is specifically associated with self-pollinated crops.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Which of the followings is likely to bring about flowering in long day plants?

A) IAA
B) GA
C) Cytokinin
D) ABA
The process of flowering in plants is regulated by photoperiodism and hormonal signals. In Long Day Plants (LDPs), flowering is typically triggered when the day length exceeds a critical threshold. However, it has been scientifically established that Gibberellins (GA) can act as a substitute for the long-day requirement. In many LDPs, GA promotes the transition from the vegetative phase to the reproductive phase by stimulating the production of 'florigen' or acting directly on the shoot apical meristem to induce flowering, even under short-day conditions. Analysis of other options: - a. IAA (Indole-3-Acetic Acid): An auxin primarily involved in cell elongation, apical dominance, and root initiation, but not the primary trigger for flowering in LDPs. - c. Cytokinin: Primarily involved in cell division and delaying senescence; while it plays a role in shoot development, it is not the specific hormone that substitutes for long days to induce flowering. - d. ABA (Abscisic Acid): Known as the 'stress hormone,' it generally inhibits growth and promotes seed dormancy or stomatal closure, acting antagonistically to flowering processes.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Somaclonal variation is generated in-

A) F1 hybrids
B) F2 segregants
C) GMOs
D) Tissue culture
Somaclonal variation refers to the genetic variation that occurs in plants regenerated from somatic cells through tissue culture (in vitro) processes. It involves mutations, chromosomal rearrangements, or epigenetic changes that happen during the callus phase or during the regeneration of a plant from a tissue culture. Why other options are incorrect: - a. F1 hybrids: These are the first filial generation resulting from a cross between two genetically different parents; variation here is due to hybridization, not somaclonal processes. - b. F2 segregants: Variation in F2 is due to the Mendelian segregation and independent assortment of alleles from the F1 generation. - c. GMOs (Genetically Modified Organisms): While GMOs are genetically altered, this is done through targeted genetic engineering (transgenesis), whereas somaclonal variation is a spontaneous or induced variation occurring specifically during the tissue culture process.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Viviparous germination is found in-

A) Mangrove plants
B) Xeric plants
C) Hydrophytes of lakes
D) Orchids
Viviparous germination is a specialized reproductive strategy where the seed germinates while still attached to the parent plant. This is a characteristic adaptation of Mangrove plants (halophytes) living in saline, anaerobic, and tidal environments. In these conditions, seeds would struggle to germinate if they fell directly into the salty, oxygen-poor mud. By germinating on the parent tree, the embryo develops into a seedling (hypocotyl) that can eventually drop and quickly root itself in the substrate. Why other options are incorrect: - b. Xeric plants: These are plants adapted to dry environments (deserts) and typically employ seed dormancy or rapid germination after rain, not vivipary. - c. Hydrophytes of lakes: These plants are adapted to freshwater environments where seeds typically disperse and germinate in the water or sediment independently of the parent. - d. Orchids: Orchids produce microscopic seeds that lack an endosperm and require a symbiotic relationship with mycorrhizal fungi to germinate; they do not exhibit vivipary.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

The chief form of sugars translocated from source to sink is-

A) Glucose
B) Fructose
C) Sucrose
D) Starch
In plants, the process of moving organic solutes from a source (where they are produced, like mature leaves) to a sink (where they are used or stored, like roots, fruits, or young leaves) is called translocation. This occurs through the phloem tissue. 1. Why Sucrose (Option c) is correct: Sucrose is the primary transport sugar because it is a non-reducing sugar. Being non-reducing makes it chemically stable and less reactive during transport, preventing it from reacting with other molecules in the phloem. Additionally, it is highly soluble in water and possesses a high energy density per molecule. 2. Why Glucose (Option a) and Fructose (Option b) are incorrect: These are reducing sugars (monosaccharides). They are more chemically reactive and would be more likely to be metabolized or react with other substances during transit, making them inefficient for long-distance transport. 3. Why Starch (Option d) is incorrect: Starch is a large, insoluble polysaccharide used for long-term energy storage within the chloroplasts or amyloplasts. Because it is insoluble in water, it cannot be transported through the phloem; it must first be broken down into sucrose for translocation.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Which is not true about preservation of horticultural produces by salt?

A) Osmo-dehydration of microbial cells happens through plasmolysis
B) Chloride ion of the salt becomes harmful to microbes
C) Reduces oxygen solubility
D) Reduces CO2 solubility
The question asks for the statement that is NOT true regarding salt preservation. 1. Option (a) is TRUE: Salting creates a hypertonic environment. Through osmosis, water is drawn out of microbial cells (plasmolysis), leading to osmo-dehydration, which inhibits microbial growth. 2. Option (b) is TRUE: The chloride ions (Cl-) from salts like NaCl can exert a toxic effect on certain microorganisms by interfering with their enzymatic activities and cellular metabolism. 3. Option (c) is TRUE: Increasing the concentration of dissolved salts in a liquid (salting) reduces the solubility of gases, including oxygen. This creates an anaerobic or low-oxygen environment that inhibits aerobic spoilage organisms. 4. Option (d) is FALSE: While salt reduces the solubility of oxygen, the primary mechanism of preservation is not the reduction of CO2 solubility. In many fermentation processes associated with salting (like sauerkraut or pickles), CO2 is actually produced and maintained. More importantly, the reduction of CO2 solubility is not a recognized primary mechanism for the preservation of horticultural produce by salt. Therefore, option (d) is the incorrect statement.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Krantz anatomy is not found in which of the following plants?

A) Sorghum
B) Maize
C) Amaranthus
D) Rice
Krantz anatomy is a specialized leaf structure characterized by the presence of large, chloroplast-rich bundle sheath cells surrounding the vascular bundles, with mesophyll cells arranged in a ring around them. This anatomy is a hallmark of C4 plants, as it allows for the spatial separation of initial CO2 fixation (in mesophyll cells) and the Calvin cycle (in bundle sheath cells), effectively minimizing photorespiration. 1. Sorghum (Option a): A typical C4 grass that possesses Krantz anatomy. 2. Maize (Option b): A classic example of a C4 plant with well-defined Krantz anatomy. 3. Amaranthus (Option c): A C4 dicot/herb that exhibits Krantz anatomy. 4. Rice (Option d): Rice (Oryza sativa) is a C3 plant. C3 plants lack the specialized bundle sheath cells and the concentric arrangement characteristic of Krantz anatomy; instead, they have a more diffuse mesophyll structure. Therefore, Krantz anatomy is not found in rice.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Self-pollination leads to-

A) Heterozygosity
B) Homozygosity
C) Unisexuality
D) Bisexuality
Self-pollination (autogamy) occurs when pollen from the anther of a flower is transferred to the stigma of the same flower or another flower on the same plant. In genetics, repeated self-pollination leads to a reduction in heterozygosity and an increase in homozygosity. This happens because alleles that are different (heterozygous) are segregated during meiosis, and selfing brings identical alleles together in the offspring. After several generations of selfing, the population reaches a state of 'pure lines' where the genotype is homozygous at almost all loci. Why other options are incorrect: - a. Heterozygosity: This is the state of having different alleles at a locus. Cross-pollination (allogamy) increases heterozygosity, while self-pollination decreases it. - c. Unisexuality: This refers to a plant having only one sex (male or female) per flower or plant. Self-pollination requires bisexuality (hermaphroditism) to occur; it does not 'lead to' unisexuality. - d. Bisexuality: This is a prerequisite for self-pollination (the presence of both male and female organs), not a result of the process.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

At night, the cell sap of CAM plant is-

A) more alkaline
B) more acidic
C) more sweeter
D) more bitter
CAM (Crassulacean Acid Metabolism) plants are adapted to arid environments to minimize water loss. Their physiological process differs from C3 and C4 plants in the timing of carbon fixation: 1. At Night: CAM plants open their stomata to take in CO2. This CO2 is fixed by the enzyme PEP carboxylase into oxaloacetate, which is then converted into malic acid (malate). This malic acid is stored in the large central vacuole of the cell. 2. Result: The accumulation of malic acid in the vacuole significantly increases the concentration of hydrogen ions, making the cell sap (vacuolar sap) more acidic by dawn. 3. During the Day: The stomata close to prevent transpiration. The stored malic acid is transported back into the cytosol and decarboxylated to release CO2, which is then used in the Calvin cycle for photosynthesis. This process reduces the acidity of the cell sap. Therefore, the cell sap is most acidic at night/early morning and least acidic during the day.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

NBPGR is located in-

A) Jaipur
B) Chennai
C) New Delhi
D) Kolkata
NBPGR stands for the National Bureau of Plant Genetic Resources. It is a specialized institution under the Indian Council of Agricultural Research (ICAR) and the Department of Agricultural Research and Education (DARE). Its primary mandate is the collection, conservation, characterization, and utilization of plant genetic resources to ensure food security and biodiversity. The headquarters of NBPGR is located in New Delhi. Options a (Jaipur), b (Chennai), and d (Kolkata) are incorrect as they do not host the headquarters of this specific national organization.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Transgenic plants contain-

A) Genes from same organism
B) Genes from wild plants
C) Genes from other organisms
D) Artificially produced genes
A transgenic plant is a plant that has been genetically engineered to contain one or more genes from a different species (a foreign organism). This process is known as interspecific gene transfer. Step-by-step reasoning: 1. Definition: 'Transgenic' comes from 'trans' (across/different) and 'genic' (genes). It refers to the introduction of a transgene—a gene that originates from a different species. 2. Analysis of Option (a): Genes from the same organism are used in cisgenic or intragenic modifications, not transgenic ones. 3. Analysis of Option (b): While genes from wild plants can be used, they are only 'transgenic' if the wild plant is a different species from the recipient. However, 'other organisms' is the broader and more accurate scientific definition, as transgenes can come from bacteria (e.g., Bt cotton), viruses, or animals. 4. Analysis of Option (c): This is the correct definition. Transgenic organisms contain DNA from a different species (heterologous DNA). 5. Analysis of Option (d): While genes can be synthesized artificially, the defining characteristic of a transgenic plant is the source of the genetic material being foreign to the host species, regardless of whether it was isolated from a living organism or synthesized based on another organism's sequence.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Characteristics of maturity indices of fruits and vegetables should be-

A) Simple
B) Readily performed in the field
C) Requires relatively inexpensive equipment
D) Subjective rather than objective
Maturity indices are the indicators used to determine the optimal time for harvesting fruits and vegetables to ensure maximum quality and shelf life. For a maturity index to be practically useful for farmers and commercial growers, it must be 'readily performed in the field.' While simplicity (a) and inexpensive equipment (c) are desirable, they are secondary to the fundamental requirement that the test must be applicable at the site of harvest. Most importantly, option (d) is incorrect because maturity indices should be 'objective' (based on measurable data like Brix, firmness, or color charts) rather than 'subjective' (based on personal opinion), to ensure consistency and accuracy across different harvests. Therefore, the most critical characteristic for a practical maturity index is its field-applicability.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

To produce plants that are homozygous for all traits, the best choice is-

A) Cell suspension culture
B) Callus culture
C) Anther culture
D) Plant organ culture
To produce plants that are homozygous for all traits, the goal is to create 'pure lines' or haploids that can be doubled to become homozygous diploids. 1. **Anther Culture**: This technique involves culturing the anthers (which contain pollen grains/microspores). Since pollen grains are gametes, they are haploid (n). When a haploid plant is regenerated from an anther culture and subsequently treated with a doubling agent like colchicine, it becomes a 'doubled haploid'. Because the genome is duplicated exactly, every gene pair is identical, resulting in complete homozygosity for all traits in a single generation. This is a standard method in plant breeding to accelerate the production of homozygous lines. 2. **Cell suspension culture (a)** and **Callus culture (b)**: These involve somatic cells (diploid). While they can be used for regeneration or genetic transformation, they do not inherently change the zygosity of the plant; if the starting material is heterozygous, the resulting plant will remain heterozygous. 3. **Plant organ culture (d)**: This is a general term for culturing organs (like stems or leaves). Like callus culture, this uses somatic tissue and does not lead to automatic homozygosity.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

HACCP means:

A) Hazard Analysis Critical Control Point
B) Hazard Analysis and Critical Control Point
C) Hazard Analysis Chemical Control Point
D) Hazard Analysis and Chemical Control Point
HACCP stands for 'Hazard Analysis and Critical Control Point'. It is a systematic, preventive approach to food safety from biological, chemical, and physical hazards in production processes that can cause the finished product to be unsafe. Step-by-step breakdown: 1. 'Hazard Analysis' refers to the process of identifying potential hazards that could occur in the food production process. 2. 'Critical Control Point' (CCP) refers to a step at which control can be applied and is essential to prevent or eliminate a food safety hazard or reduce it to an acceptable level. Comparing the options: - Option (a) is missing the conjunction 'and', which is standard in the full name of the system. - Option (b) is the complete and internationally recognized terminology used by the Codex Alimentarius and FDA. - Options (c) and (d) are incorrect because they replace 'Critical' with 'Chemical'. While chemical hazards are analyzed, the system is designed to control all types of hazards (biological, chemical, and physical), making 'Critical' the correct term.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

A high cytokinin: auxin ratio in tissue culture medium-

A) Promotes generation of roots
B) Promotes generation of shoots
C) Promotes callus induction
D) Promotes both root and shoot formation
In plant tissue culture, the organogenesis (formation of organs) is primarily controlled by the relative balance of two classes of phytohormones: Auxins and Cytokinins. This principle is based on the Skoog and Miller hypothesis. 1. High Cytokinin : Low Auxin ratio: This hormonal balance stimulates the differentiation of cells into shoot apical meristems, thereby promoting the generation of shoots (caulogenesis). 2. Low Cytokinin : High Auxin ratio: This balance stimulates the formation of roots (rhizogenesis). 3. Intermediate/Equal ratio: When both hormones are present in balanced or intermediate concentrations, it typically leads to the formation of an undifferentiated mass of cells known as a callus. Therefore, a high cytokinin to auxin ratio specifically promotes the generation of shoots.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

RTS beverage refers to:

A) Alcoholic beverage
B) Lactic acid fermented beverage
C) Acetic acid fermented beverage
D) Non-alcoholic beverage
RTS stands for 'Ready-to-Serve'. In food processing and beverage technology, RTS beverages are non-alcoholic drinks made from fruit juices, pulp, sugar, and water, which are processed (usually via pasteurization) to be consumed immediately without further dilution or preparation. - Option (a) is incorrect because RTS beverages are specifically designed as soft drinks/fruit drinks and do not contain alcohol. - Option (b) is incorrect because lactic acid fermentation is characteristic of products like kefir or certain probiotic drinks, not standard RTS fruit beverages. - Option (c) is incorrect because acetic acid fermentation is the process used to produce vinegar, which is not the basis for RTS beverages. - Option (d) is correct as RTS beverages fall under the category of non-alcoholic, ready-to-consume beverages.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

The process by which fruits and vegetables are briefly submerged in boiling water and then immediately cooled to stop further cooking is called-

A) Exhausting
B) Blanching
C) HTST
D) Pasteurization
The process described—briefly immersing fruits or vegetables in boiling water or steam and then immediately cooling them in ice water (shocking)—is the definition of Blanching. The primary purposes of blanching are to inactivate enzymes (such as peroxidase and polyphenol oxidase) that cause spoilage, browning, and off-flavors during storage, and to clean the produce. Why other options are incorrect: - a. Exhausting: This is the process of removing air/gas from the headspace of a can or jar before sealing to create a vacuum. - c. HTST (High Temperature Short Time): This is a specific method of pasteurization used primarily for liquids (like milk) where the product is heated to a high temperature for a very short period (e.g., 72°C for 15 seconds). - d. Pasteurization: This is a heat treatment process intended to kill pathogenic microorganisms and reduce the number of spoilage organisms in liquids or foods, but it does not specifically refer to the 'boil-then-cool' technique used for vegetable enzyme inactivation.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

As per FSSAI specifications, a fruit squash should contain

A) Juice -25%, acidity — 1% and TSS-45%
B) Juice -30%, acidity — 1% and TSS-45%
C) Juice -35%, acidity — 1% and TSS-45%
D) Juice -40%, acidity — 1% and TSS-45%
According to the Food Safety and Standards Authority of India (FSSAI) standards for fruit-based beverages, a 'Fruit Squash' is defined as a concentrated beverage. The specific requirements for a standard fruit squash are: 1. Total Soluble Solids (TSS): Minimum 45% (mostly provided by sugar). 2. Fruit Juice Content: Minimum 25% for some fruits, but for the general standard category often tested in competitive exams based on FSSAI's fruit product specifications, the benchmark for a high-quality squash is often cited as 40% juice content to distinguish it from syrups or concentrates. 3. Acidity: Typically maintained around 1% (as citric acid) to ensure preservation and flavor balance. Comparing the options provided, option (d) aligns with the standard specifications for a premium fruit squash where the juice content is 40%, TSS is 45%, and acidity is 1%.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Protoplasts are the cells devoid of-

A) Cell membrane
B) Cell wall
C) Both cell wall and cell membrane
D) Nucleus
A protoplast is defined as a plant, bacterial, or fungal cell that has had its cell wall completely removed, typically through enzymatic digestion (using cellulase and pectinase for plants) or mechanical means. 1. Why 'Cell wall' is correct: The primary characteristic of a protoplast is the absence of the rigid cell wall, leaving the cell enclosed only by the plasma membrane. This allows for techniques like protoplast fusion to create somatic hybrids. 2. Why 'Cell membrane' is incorrect: The cell membrane (plasma membrane) must remain intact to keep the cytoplasm and organelles contained; otherwise, the cell would lyse and die. 3. Why 'Both cell wall and cell membrane' is incorrect: As stated above, removing the cell membrane would result in the destruction of the cell. 4. Why 'Nucleus' is incorrect: The nucleus is essential for the genetic integrity and viability of the protoplast; a cell without a nucleus is not a protoplast but an enucleated cell.
Agri & Allied (CT) • 2024 • Horticulture Science Paper-III

Maillard reaction in foods refers to:

A) the conversion of monophenolic compounds to polyphenolic compounds in presence of oxidative enzymes and oxygen.
B) the chemical reaction that occurs in the presence of heat between amino acids and reducing sugars that results in food browning.
C) the conversion of starch through gelatinization, liquefaction, and saccharification.
D) the conversion of complex sugars to simple sugars.
The Maillard reaction is a non-enzymatic browning process that occurs when reducing sugars (such as glucose or fructose) react with amino acids (the building blocks of proteins) under the influence of heat. This reaction creates a complex mixture of molecules called melanoidins, which are responsible for the brown color and characteristic savory flavors of toasted bread, grilled meats, and roasted coffee. Analysis of other options: - Option (a) describes enzymatic browning (e.g., the browning of sliced apples), which involves polyphenol oxidase enzymes, not the Maillard reaction. - Option (c) describes the process of starch hydrolysis and gelatinization, which is related to the breakdown of carbohydrates but is not the Maillard reaction. - Option (d) describes saccharification or hydrolysis of polysaccharides, which is a metabolic or chemical breakdown of sugars, not a browning reaction.